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Section: General Biotechnology / Microbial Biotechnology
 
     
 
Microbial Biotechnology
 
   
 
Content

Features of Biotechnological Importance in Microorganisms

Ä Fermentation

Ä Microorganisms

Ä Cultures of microorganism

 

Ä Solid or semisolid culture

 

Ä Batch culture

 

Ä Continuous culture

 

Ä Fed-batch culture

Ä Metabolic pathways in microorganisms

 

Ä Glycolysis or EMP pathway

 

Ä The Entner-Doudoroff pathway

 

Ä The pentose phosphate pathway

Ä Microbial products

 

Ä Primary metabolites

 

Ä Secondary metabolites

 

Ä Enzymes

 

Ä Microbial biomass


Primary Metabolites

Ä Vitamins

 

Ä Vitamins B12
    Ä Chemical structure
    Ä Commercial production
Ä Organic acids

 

Ä Citric acid

 

 

Ä Commercial production

 

 

Ä Biochemistry of fermentation

 

Ä Use of organic acids

Ä Alcohols

 

Ä Microorganism used in alcohol production

 

Ä Fermentable substrates

 

Ä Biochemistry of fermentation

 

 

Ä Ethanol fermentation by yeasts

 

 

Ä Ethanol fermentation by bacteria

 

Ä Ethanol fermentation methods

 

Ä Distilleries producing alcohols

 

Ä Alcoholic beverages

 

 

Ä Wine

 

 

Ä Beer

 

 

Ä Rum

 

 

Ä Whisky

 

 

Ä Sake

 

Ä Uses of alcohols



Secondary Metabolites

Ä Toxins

 

Ä Bacterial toxins

 

 

Ä Chemical nature

 

 

ÄProduction of b-exotoxin

 

 

Ä Microbial insecticides

 

Ä Mycotoxins, type, action, control

Ä Antibiotics

 

Ä Penicillins

 

 

Ä Selection of culture of penicillium

 

 

Ä Chemical nature of penicillins

 

 

Ä Fermentation medium

 

 

Ä Fermentation process

 

 

Ä Antibiotic producing companies


Enzyme Technology

Ä Microorganisms

Ä Properties of enzymes

 

Ä Presence of species specificity

 

Ä Variation in activity and stability

 

Ä Substrate specificity

 

Ä Activation and inhibition

Ä Methods of enzyme production

 

Ä Isolation of microorganisms, strain development and preparation of inoculum 

 

Ä Medium formulation and preparation

 

Ä Sterilization and inoculation of medium, maintenance of culture and fluid filtration

 

Ä Purification of enzymes

Ä Immobilization of enzymes

 

Ä Advantages of using immobilized enzymes

 

Ä Methods of enzyme immobilization

 

 

Ä Adsorption

 

 

Ä Covalent bonding (Ionic bonding)

 

 

Ä Entrapping

 

 

Ä Cross linking

 

 

Ä Encapsulation

 

Ä Effects of enzyme immobilization on enzyme stability

Ä Enzyme engineering

Ä Application of enzymes

 

Ä Therapeutic uses

 

Ä Analytical uses

 

Ä Manipulative uses

 

Ä Industrial uses

 

 

Ä In dairy industry

 

 

Ä In detergent industry

 

 

Ä In starch industry

 

 

Ä In brewing industry

 

 

Ä In wine industry

 

 

Ä In pharmaceutical industry

Ä Biosensor

 

Ä Types of biosensor

 

Ä Applications of biosensor

Ä Biochips

 

Ä Principles of Biochips

 

Ä Application of Biochips




Single Cell Protein (SCP) and Mycoprotein

Ä Advantages of producing microbial protein

Ä Microorganisms use as single cell protein (SCP)

Ä Substrates used for the production of SCP

Ä Nutritional values of SCP

Ä Genetic improvements of microbial cells

Ä Production of algal biomass

 

Ä Factors affecting biomass production

 

Ä Harvesting the algal biomass

 

Ä Spirulina as SCP, cultivation and uses

Ä Production of bacterial and actinomycetous biomass

 

Ä Method of production

 

Ä Factors affecting biomass production

 

Ä Product recovery

Ä Production of yeast biomass

 

Ä Factors affecting growth of yeast

 

Ä Recovery of yeast biomass

Ä Production of fungal biomass (Other than Mushrooms)

 

Ä Growth conditions

 

Ä Organic wastes as substrates

 

Ä Traditional fungal foods

 

 

Ä Shoyu

 

 

Ä Miso

 

 

Ä Sake

 

 

Ä Tempeh  

Ä Mushroom culture

 

Ä Historical background

 

Ä Present status of mushroom culture in India

 

Ä Nutritional values

 

Ä Cultivation methods

 

 

Ä Obtaining pure culture 

 

 

Ä Preparation of spawns

 

 

Ä Formulation and preparation of composts

 

 

Ä Spawning, spawn running and cropping

 

Ä Control of pathogens and pests

 

Ä Cultivation of paddy straw mushroom

 

Ä Cultivation of white button mushroom

 

Ä Cultivation of Dhingri (Pleurotus sajor-caju)

 

Ä Recipes of mushroom


 
     
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