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Section: General Biotechnology / Plant Biotechnology
 
     
 
In Vitro Culture Techniques : The Biotechnological Principles
 
   
 

During the last two decades plant cell, tissue and organ culture have developed rapidly and become a major biotechnological tool in agriculture, horticulture, forestry and industry. Those problems which were not feasible through conventional techniques, now have been solved via these techniques, for example, inter- and intra-specific crosses, micropropagation, somaclonal variation, encapsulated seeds, etc.

To boost up these areas ICGEB in a workshop (held at New Delhi from September 18 to 20, 1985), recommended the need of more research in developing countries on plant cell culture, differentiation, regeneration and transformation in tropical grain legumes, woody legumes and cereals. The emphasis was laid to improve growth under stress condition, pest and disease resistance, improved nutritional quality, nitrogen fixation and the control of partitioning within the plants. A detailed account of plant cells, tissue and organ culture is given in this context.

Content

Ä Totipotency

Ä Historical background

Ä Requirements for cell and Tissue Cultures

 

Ä A tissues culture laboratory

 

Ä Nutrient media

 

 

Ä Inorganic chemicals

 

 

Ä Growth hormones

 

 

Ä Organic constitutents

 

 

Ä Vitamins

 

 

Ä Amino acids

Ä Culture of plant materials

 

Ä Explant culture

 

Ä Callus formation and its culture

 

Ä Organogenesis

 

Ä Root culture

 

Ä Shoot culture and micropropagation

 

Ä Cell culture

 

 

Ä Benefits from cell culture

 

Ä Somatic embryogenesis

 

Ä Somaclonal variation

 

Ä Protoplast culture

 

 

Ä Isolation

 

 

Ä Regeneration

 

Ä Protoplast fusion and somatic hybridization

 

 

Ä Fusion products

 

 

Ä Method of somatic hybridization

 

Ä Anther and pollen Culture

 

 

Ä Culturing techniques

 

Ä In vitro androgenesis (direct and indirect androgenesis)

 

Ä Mentor pollen technology

 

Ä Embryo culture

 

Ä Embryo rescue

 

Ä Protoplast fusion in fungi




Totipotency: The Basis of Plant Cell and Tissue Culture
Each living cell, of a multicellular organism, is capable of independent development, when provided with suitable conditions (White, 1963). Morgan (1901) coined the term 'totipotency' to denote this capacity of cell to develop into an organism by regeneration. However, the concept of totipotency is important in tissue culture. Use of multicellular organisms in research, as biological units, is rather difficult; therefore, attempts to study an organism by reducing to its constituent cells and subsequently the cultured cells as basic organism, are of fundamental importance (White, 1963).


 
     
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