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Section: General Biotechnology / Biotechnology & Environment
 
     
 
Environmental Biotechnology
 
   
 
The area of polluted soil and water is expanding day by day due to rapid increase in world population. About 100 million people are added to the world population each year. Therefore, more and more industries, food, health care, medicines, vehicles, etc. are required to meet the demand of people. This is possible by involving biotechnology. However, it is probable that the modern biotechnology based on the sophisticated art of genetic engineering could solve all these problems.

The environmental biotechnology employs the application of genetic engineering to improve the efficiency and cost which are central to the future widespread exploitation of microorganisms to reduce the environmental burden of toxic substances. It is hoped that in future the application of microorganisms coupled with genetic engineering techniques will make a major contribution to improve the quality of our environment. However, is there risk associated with release of genetically engineered microorganisms into the environment!

Uses of microorganisms in environmental clean up with special reference to bioremediation types, processes and methods with several examples (e.g. hydrocarbons, heavy metals, dyes, xenobiotics, etc), utilization of sewage and agro-wastes, and the benefits and hazards associated with release of GMMs for our clean environment have been discussed in this section. The benefits expected from the release of GMMs into the environment are summarized in Table 21.1.

Table 21.1. Benefits from release of genetically modified microorganisms into the environment.
A. Protection of environment
      - Bioremediation of polluted environment
B. Control of global environmental process
      - Reversal of land desertification
      - Reversal of green house effect
C. Agriculture

      - Increasing efficiency of plant nutrition
      - Pest control (safe biopesticides)
      - Protection of plants from climatic stress
      - Protection of plants from tumor formation and disease
D. Food Industry
      - Microorganisms producing enzymes for food industry
      - Microorganisms with improved efficiency of fermentation
      - Improved microorganisms for milk industry
E.  Health care
- Microorganisms as live attenuated vaccines
Source: Velkov (1996).


Content

Ä Bioremediation

 

Ä In situ bioremediation

 

 

Ä Intrinsic bioremediation

 

 

Ä Engineered in situ bioremediation

 

Ä Ex situ bioremediation

 

 

Ä Solid phase system (composting, composting process)

 

 

Ä Slurry phase system (aerated laggons, low shear airlift reactor)

 

 

Ä Factors affecting slurry phase bioremediation

 

Ä Bioremediation of hydrocarbon

 

 

Ä Use of mixture of bacteria

 

 

Ä Use of genetically engineered bacterial strains 

 

Ä Bioremediation of Industrial wastes

 

 

Ä Bioremediation of dyes

 

 

Ä Bioremediation of heavy metals

 

 

Ä Bioremediation of coal waste through VAM fungi

 

Ä Bioremediation of xenobiotics

 

 

Ä Microbial degradation of xenobiotics

 

 

Ä Gene manipulation of pesticide-degrading microorganisms

Ä Utilization of sewage, and agro-wastes

 

Ä Production of single cell protein

 

Ä Biogas from sewage

 

Ä Mushroom production on agro-wastes

 

Ä Vermicomposting

Ä Microbial leaching (bioleaching)   

 

Ä Microorganisms used in leaching

 

Ä Chemistry of leaching

 

 

Ä Direct leaching

 

 

Ä Indirect leaching

 

Ä Leaching process (slope leaching heap leaching in situ leaching)

 

Ä Examples of bioleaching

 

 

Ä Copper leaching

 

 

Ä Uranium leaching

 

 

Ä Gold and silver leaching

 

 

Ä Silica leaching

Ä Hazards of environmental engineering

 

Ä Survival of released GMMs in the environment

 

 

Ä Adaptive mutagenesis in GMMs

 

 

Ä Gene transfer from GMMs into other microorganisms

 

 

Ä Gene transfer via conjugative transposons

 

 

Ä Effect of environmental factors on gene transfer

 

Ä Ecological impact of GMMs released into the environment

 

 

Ä Growth inhibition of natural strains

 

 

Ä Growth stimulation of indigenous strains

 

 

Ä Replacement of natural strains

 

Ä Monitoring of GEMs in the environment

 

 

Ä Risk assessment of the GEMs released into the environment


 
     
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