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Section: General Biotechnology / Animal Biotechnology
 
     
 
Animal Biotechnology
 
   
 

Content

Animal Cell, Tissue and Organ Culture

 

Ä Requirements for animal cell, tissue and organ culture

 

 

Ä Substrates for cell culture

 

 

Ä Substrate treatment

 

 

Ä Culture media

 

 

 

Ä Natural media

 

 

 

Ä Synthetic media

 

 

Ä Sterilization of glassware, equipments and culture media

 

 

Ä Isolation of animal material (tissue)

 

 

 

Ä Disaggregation of tissue (physical and enzymatic methods)

 

 

 

Ä Establishment of cell culture

 

Ä Cultivation of animal cell en masse in bioreactor

 

Ä Immobilized cell culture

 

Ä Insect cell culture

 

Ä Somatic cell culture

 

Ä Organ culture

 

 

Ä Organ culture on plasma clots

 

 

Ä Organ culture on agar

 

 

Ä Organ culture in liquid medium

 

 

Ä Whole embryo culture

 

Ä Valuable products from cell cultures

 

 

Ä Monoclonal antibodies

 

 

Ä Production of commercial products from insect culture

 

 

 

 

Manipulation of Reproduction and Transgenic Animals

 

Ä Manipulation of reproduction in animals

 

 

Ä Artificial insemination

 

 

 

Ä Semen collection and its storage

 

 

 

Ä Ovulation control

 

 

 

Ä Sperm sexing

 

 

Ä Embryo transfer

 

 

 

Ä Multiple ovulation (superovulation)   

 

 

 

Ä Multiple ovulation with embryo transfer

 

 

 

Ä Embryo splitting

 

 

 

Ä Embryo sexing

 

 

Ä In vitro fertilization (IVF) technology

 

 

 

Ä In vitro Maturation (IVM) of oocytes

 

 

 

Ä Culture of in vitro fertilized embryos

 

 

Ä Embryo Cloning

 

 

 

Ä Quadriparental hybrid

 

 

 

Ä Nuclear trans­ plantation (Dolly)

 

 

 

Ä Embryonic stem cells

 

Ä In vitro fertilization and embryo transfer in humans

 

 

Ä Infertilities in humans

 

 

 

Ä Male sterility

 

 

 

Ä Female sterility

 

 

Ä Who benefits IVF

 

 

Ä How the patients for IVF treated

 

 

 

Ä Indicators of ovary stimulation

 

 

 

Ä Oocyte recovery and uptake

 

 

 

Ä Semen preparation

 

 

Ä IVF and embryo transfer

 

Ä Transgenic animals

 

 

Ä Strategies for gene transfer

 

 

 

Ä Transfer of animal cells/embryo

 

 

 

Ä Treatment through microinjection

 

 

 

Ä Targeted gene transfer

 

 

Ä Transgenic animals

 

 

Ä Transgenic sheep

 

 

Ä Transgenic fish

 

 

Ä Animal bioreactor and molecular farming

 

Ä Application of molecular genetics

 

 

Ä Selected traits and their breeding into livestock

 

 

 

Ä Diagnosis, elimination and breeding strategies of genetic diseases

 

 

Ä Application of molecular genetics in improvement of livestock

 

 

 

Ä Hybridization based markers

 

 

 

Ä PCR-based markers

 

 

 

Ä Properties of molecular markers

 

 

 

Ä Application of molecular markers

 

 

 

Ä Transgenic breeding strategies




 
     
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