Nitrate Reductase
Nitrate + reduced pyridine nucleotides (NADH, NADPH) → nitrite + oxidized pyridine nucleotides (NAD⁺, NADP⁺)

Nitrate reduction requires molybdenum as a cofactor. A two-electron transfer takes place to reduce nitrate (N oxidation state, +5) to nitrite (N oxidation state, +3). Respiration is the likely source of reduced pyridine nucleotides in roots and also, along with photosynthesis, can be a source in shoots. The conversion of nitrite into ammonia is mediated by nitrite reductase, which is located in the chloroplasts of green tissues and in the proplastids of roots and nongreen tissues (5,7,8).


Nitrite Reductase
Nitrite + reduced ferredoxin → ammonium + oxidized ferredoxin

In leaves, nitrite reduction involves the transfer of six electrons in the transformation of nitrite to ammonium. No intermediates, such as hyponitrous acid (H₂N₂O₂) or hydroxylamine (HONH₂), are released, and the reduction takes place in one transfer. The large transfer of energy and reducing power required for this reaction is facilitated by the process being located in the chloroplasts (8). In roots, a ferredoxin-like protein may function, and the energy for producing the reducing potential is provided by glycolysis or respiration (9,10).

In plants, roots and shoots are capable of nitrate metabolism, and the proportion of nitrate reduced in roots or shoots depends on plant species and age, nitrogen supply, temperature, and other environmental factors (11–15). The assimilation of nitrate is an energy-consuming process, using the equivalent of 15 mol of adenosine triphosphate (ATP) for each mole of nitrate reduced (16). The assimilation of ammonia requires an additional five ATP per mole. In roots, as much as 23% of the respiratory energy may be used in nitrate assimilation compared with 14% for ammonium assimilation (17). However, nitrate can be stored in cells without toxic effects, but ammonium is toxic at even low concentrations and must be metabolized into organic combination. Consequently, ammonium metabolism for detoxification may deplete carbon reserves of plants much more than nitrate accumulation.