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Section: Genetics » Regulation of Gene Expression » Operon Circuits in Prokaryotes
 
 
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  Pribnow box and other sequences common to DNA regions upstream to several operons
 
     
 
Content
⇒ Regulation of Gene Expression 1.  Operon Circuits in Bacteria and other Prokaryotes
⇒ Induction and repression
⇒ Inducer and co-repressor
⇒ The operon model for transcriptional regulation 
⇒ The tryptophan operon in bacteria (E. coli and Salmonella)
⇒ Tryptophan (trp) repressor controls three sets of genes
⇒ Negative and Positive Controls of Transcription
⇒ Substitution of Sigma Factor and Control of Transcription
⇒ Multiple sigma factors in E. coli 
⇒ Sporulation in bacteria
⇒ DNA sequences controlling transcription 
⇒ DNA sequences for CAP, RNA polymerase and lac-repressor
⇒ Identification of starting point
⇒ Pribnow box and other sequences common to DNA regions upstream to several operons
⇒ Regulation by DNA rearrangements
⇒ Post-transcriptional regulation
⇒ Leader sequences and attenuators
⇒ Autogenous regulation of translation
⇒ Regulation by alternative splicing
⇒ Regulation by-anti-sense RNA
⇒ Repression and activation of translation
⇒ Feedback inhibition
⇒ Signal transduction and ‘two component regulatory system’
Pribnow box and other sequences common to DNA regions upstream to several operons
Upstream to starting point of lac operon are number of sequences which are common to many E. coli and bacteriophage genes. There is a sequence at about 10 bases upstream from starting point (also called minus ten or - 10 sequence) called Pribnow box which is an important sequence and named after D. Pribnow, who first discovered it in 1975.

Pribnow box is shown in Figure 35.21 and has the following sequence in lac operon.

This sequence differs slightly from the consensus sequence for Pribnow box (TATAAT) described in the previous topic, where it was shown that among prokaryotes the range of conservation of bases varies from 45% to 96%. There are other common sequences located at -30 and -60 bases (upstream), the latter being CAP site, which is marked by an interrupted three base pair inverted repeat, flanked by non-inverted T-A base pair on either side as follows.


This CAP binding site is common to lac,gal and ara operons. It lies within -53 to -23 bp in gal operon,bctween -72 to -52bp in lac operon and between -107 to -78bp in ara operon.


 
     






     
     
 
 
     
 
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