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Section: Genetics » Regulation of Gene Expression » Operon Circuits in Prokaryotes
 
 
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  DNA sequences for CAP, RNA polymerase and lac-repressor
 
     
 
Content
⇒ Regulation of Gene Expression 1.  Operon Circuits in Bacteria and other Prokaryotes
⇒ Induction and repression
⇒ Inducer and co-repressor
⇒ The operon model for transcriptional regulation 
⇒ The tryptophan operon in bacteria (E. coli and Salmonella)
⇒ Tryptophan (trp) repressor controls three sets of genes
⇒ Negative and Positive Controls of Transcription
⇒ Substitution of Sigma Factor and Control of Transcription
⇒ Multiple sigma factors in E. coli 
⇒ Sporulation in bacteria
⇒ DNA sequences controlling transcription 
⇒ DNA sequences for CAP, RNA polymerase and lac-repressor
⇒ Identification of starting point
⇒ Pribnow box and other sequences common to DNA regions upstream to several operons
⇒ Regulation by DNA rearrangements
⇒ Post-transcriptional regulation
⇒ Leader sequences and attenuators
⇒ Autogenous regulation of translation
⇒ Regulation by alternative splicing
⇒ Regulation by-anti-sense RNA
⇒ Repression and activation of translation
⇒ Feedback inhibition
⇒ Signal transduction and ‘two component regulatory system’
DNA sequences for CAP, RNA polymerase and lac-repressor
Entire DNA segment has been sequenced from transcription termination (downstream) end of 'lac I' gene to beyond the transcription start of Z, Y, A genes of lac operon. This sequence contains the promoter and operator segments. Different parts of this sequence have been studied for their functions. For instance, sequences of three binding sites for CAP, RNA polymerase enzyme and 'lac' repressor could be identified through the technique of DNA footprinting. These sequences are shown in Fig. 35.21.

 
     






     
     
 
 
     
 
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