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Section: Genetics » Genetic Engineering and Biotechnology » Recombinant DNA and PCR
 
 
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  Construction of chimeric DNA
 
     
 
Content
⇒ Genetic Engineering and Biotechnology 1.  Recombinant DNA and PCR (Cloning and Amplification of DNA)
⇒ Restriction enzymes in cloning
⇒ Techniques used in recombinant DNA 
⇒ Cloning vectors for recombinant DNA
⇒ Plasmids as vectors
⇒ Bacteriophages as vectors
⇒ Plant and animal viruses as vectors
⇒ Transposons as vectors
⇒ Artificial chromosome vectors for cloning large DNA segments
⇒ Construction of chimeric DNA
⇒ Palindromes and staggered cleavage
⇒ Adding poly dA at the 3' ends of the vector and poly dT at the 3' ends of DNA clone
⇒ Blunt end ligation by T4 DNA ligase
⇒ Cloning in bacteria and eukaryotes
⇒ Cloning in bacteria
⇒ Cloning in eukaryotes
⇒ Molecular probes 
⇒ Labelling of probes
⇒ Applications of molecular probes
⇒ Construction and screening of genomic and cDNA libraries
⇒ Gene amplification : PCR and its applications
⇒ cDNA library from mRNA
⇒ Colony (or plaque) hybridization for screening of libraries
⇒ Gene Amplification : PCR and Its Applications
⇒ The basic polymerase chain reaction (PCR)
⇒ Different schemes of PCR
Construction of Chimeric DNA
Chimeric DNA molecules are produced by inserting a foreign DNA segment into a DNA molecule of a vector. These chimeric DNA molecules are the means of cloning genes or other small DNA sequences. The construction of chimeric DNA, as we know now, involves joining of the broken ends of the vector with the two ends of the sequence to be cloned. There are atleast three methods available for the construction of this chimeric DNA.

 
     






     
     
 
 
     
 
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