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Section: Genetics » Genetic Engineering and Biotechnology » Recombinant DNA and PCR
 
 
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  Applications of molecular probes
 
     
 
Content
⇒ Genetic Engineering and Biotechnology 1.  Recombinant DNA and PCR (Cloning and Amplification of DNA)
⇒ Restriction enzymes in cloning
⇒ Techniques used in recombinant DNA 
⇒ Cloning vectors for recombinant DNA
⇒ Plasmids as vectors
⇒ Bacteriophages as vectors
⇒ Plant and animal viruses as vectors
⇒ Transposons as vectors
⇒ Artificial chromosome vectors for cloning large DNA segments
⇒ Construction of chimeric DNA
⇒ Palindromes and staggered cleavage
⇒ Adding poly dA at the 3' ends of the vector and poly dT at the 3' ends of DNA clone
⇒ Blunt end ligation by T4 DNA ligase
⇒ Cloning in bacteria and eukaryotes
⇒ Cloning in bacteria
⇒ Cloning in eukaryotes
⇒ Molecular probes 
⇒ Labelling of probes
⇒ Applications of molecular probes
⇒ Construction and screening of genomic and cDNA libraries
⇒ Gene amplification : PCR and its applications
⇒ cDNA library from mRNA
⇒ Colony (or plaque) hybridization for screening of libraries
⇒ Gene Amplification : PCR and Its Applications
⇒ The basic polymerase chain reaction (PCR)
⇒ Different schemes of PCR
Applications of molecular probes
Molecular probes can be used for both basic and applied studies in the field of molecular biology and biotechnology. These uses, to be discussed briefly in the different topics of genetics on ePlantScience.com at appropriate places, include the following: (i) 'Restriction fragment length polymorphisms (RFLPs)' and their uses for preparation of molecular maps and other purposes (Genetic Engineering and Biotechnology 2.  Restriction Maps and Molecular Genetic Maps). (ii) Screening of genomic and cDNA libraries for isolation of gene's etc. (see later in the topic), (iii) Location of genes or DNA sequences on chromosomes, through in situ hybridization or ISH (see Organization of Genetic Material 2.  Repetitive and Unique DNA Sequences). (iv) Use in diagnosis of diseases and DNA fingerprinting (Human Genetics).

 
     






     
     
 
 
     
 
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