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Section: Genetics » Genetic Engineering and Biotechnology » Isolation, Sequencing and Synthesis of Genes
 
 
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  Synthesis of genes
 
     
 
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⇒ Genetic Engineering and Biotechnology 3.  Isolation, Sequencing and Synthesis of Genes
⇒ Isolation of genes 
⇒ Early attempts for isolation of ribosomal RNA genes in Xenopus
⇒ Isolation of genes coding for known specific proteins
⇒ Isolation of genes coding for an unknown product
⇒ Sequencing of genes or a DNA segment
⇒ Maxam and Gilbert's chemical degradation method
⇒ Synthesis of genes 
⇒ Chemical synthesis of genes
⇒ Synthesis of gene for yeast alanyl tRNA
⇒ Synthesis of gene for a true precursor tRNA
⇒ Gene synthesis machines
Synthesis of Genes
There are two approaches available for the synthesis of genes : (a) When the detailed structure of a gene is available, this gene can be synthesized by a purely chemical method as done by Khorana for the synthesis of gene for a tRNA (reported for the first time in 1970). (b) If the detailed nucleotide sequence of the gene is not available one may utilize the 'RNA directed DNA polymerase' enzyme for the synthesis of the gene in question in the form of complementary DNA (cDNA) from the mRNA of the gene. As discussed above, cDNA can also be used as a molecular probe for isolation of a gene either (i) from DNA extracted from living cells utilizing the technique of Southern blot hybridization, or from (ii) genomic library.

 
     






     
     
 
 
     
 
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