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Section: Genetics » Genetic Engineering and Biotechnology » Isolation, Sequencing and Synthesis of Genes
 
 
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  Sequencing of genes or a DNA segment
 
     
 
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⇒ Genetic Engineering and Biotechnology 3.  Isolation, Sequencing and Synthesis of Genes
⇒ Isolation of genes 
⇒ Early attempts for isolation of ribosomal RNA genes in Xenopus
⇒ Isolation of genes coding for known specific proteins
⇒ Isolation of genes coding for an unknown product
⇒ Sequencing of genes or a DNA segment
⇒ Maxam and Gilbert's chemical degradation method
⇒ Synthesis of genes 
⇒ Chemical synthesis of genes
⇒ Synthesis of gene for yeast alanyl tRNA
⇒ Synthesis of gene for a true precursor tRNA
⇒ Gene synthesis machines
Sequencing of Gene or a DNA segment
Once a gene or a DNA fragment has been cloned, its further study involves DNA sequencing. The technique of DNA sequencing was very laborious, till 1975, when a breakthrough was made in DNA sequencing methods. Two different methods are now routinely used for determination qf DNA sequences.

 
     






     
     
 
 
     
 
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