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Section: Genetics » Genetic Engineering and Biotechnology » Isolation, Sequencing and Synthesis of Genes
 
 
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  Isolation of genes
 
     
 
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Genetic Engineering and Biotechnology 3.  Isolation, Sequencing and Synthesis of Genes
Isolation of genes 
Early attempts for isolation of ribosomal RNA genes in Xenopus
Isolation of genes coding for known specific proteins
Isolation of genes coding for an unknown product
Sequencing of genes or a DNA segment
Maxam and Gilbert's chemical degradation method
Synthesis of genes 
Chemical synthesis of genes
Synthesis of gene for yeast alanyl tRNA
Synthesis of gene for a true precursor tRNA
Gene synthesis machines
Isolation of Genes
During 1970s and 1980s, significant progress was made in the techniques for isolation of a variety of genes, including those for (i) ribosomal RNA; (ii) specific protein products; (iii) phenotypic traits with unknown gene product, and those for (iv) regulatory functions e.g. promoter genes, etc. Different techniques have been used for the isolation of these different types of genes and will be briefly described.


 
     






     
     
 
 
     
 
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