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Section: Genetics » Genetic Engineering and Biotechnology » Isolation, Sequencing and Synthesis of Genes
 
 
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  Genetic Engineering and Biotechnology 3. Isolation, Sequencing and Synthesis of Genes
 
     
 
Content
⇒ Genetic Engineering and Biotechnology 3.  Isolation, Sequencing and Synthesis of Genes
⇒ Isolation of genes 
⇒ Early attempts for isolation of ribosomal RNA genes in Xenopus
⇒ Isolation of genes coding for known specific proteins
⇒ Isolation of genes coding for an unknown product
⇒ Sequencing of genes or a DNA segment
⇒ Maxam and Gilbert's chemical degradation method
⇒ Synthesis of genes 
⇒ Chemical synthesis of genes
⇒ Synthesis of gene for yeast alanyl tRNA
⇒ Synthesis of gene for a true precursor tRNA
⇒ Gene synthesis machines
Molecular manipulation of specific genes is one of the very popular areas of research in biotechnology. These genes, therefore, should be either isolated or artificially synthesized before they are manipulated and used for transformation leading to the production of transgenic plants and animals that are discussed in the next topic. Before, we discuss production and uses of these transgenic plants and animals, the gene technology available for isolation, sequencing and synthesis of specific genes should be discussed. This technology will be briefly described in this section.

 
     






     
     
 
 
     
 
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