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Section: Genetics » Expression of Gene » Translation in Prokaryotes and Eukaryotes
 
 
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  Elongation of polypeptide
 
     
 
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⇒ Expression of Gene : Protein Synthesis 4. Translation in Prokaryotes and Eukaryotes
⇒ Formation of amino-acyl tRNA
⇒ Initiation of polypeptide 
⇒ Initiation in prokaryotes
⇒ Initiation in eukaryotes
⇒ Kozak's scanning hypothesis
⇒ Elongation of polypeptide
⇒ Binding of AA-tRNA at site 'A' of ribosome
⇒ Formation of peptide bond
⇒ Translocation of peptidyl tRNA from 'A' to 'P' site
⇒ Termination of polypeptide
⇒ Modification, folding and transport of released polypeptide
⇒ Translation in chloroplasts and mitochondria
Elongation of Polypeptide
In E. coli, after the formation of 70S-mRNA-f-met-tRNAfmet complex, elongation of polypeptide chain is brought about by the regular addition of amino acids. At 37°C about 15 amino acids per second are added to a growing polypeptide chain, so that in 20 seconds a protein with 300 amino acids can be synthesized. The following three steps are important in the elongation process.



 
     






     
     
 
 
     
 
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