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Section: Genetics » Chemistry of the Gene » Synthesis, Modification and Repair of DNA
 
 
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  RNA primers in DNA replication
 
     
 
Content
⇒ Chemistry of the Gene 2.  Synthesis, Modification and Repair of DNA
⇒ DNA replication: general features 
⇒ Semi-conservative DNA replication in E. coli
⇒ Semi-conservative replication of chromosomes in eukaryotes
⇒ Semi-discontinuous DNA replication
⇒ Unidirectional and bidirectional DNA replication
⇒ RNA primers in DNA replication
⇒ Regulation of DNA replication by anti-sense RNA primer
⇒ Prokaryotic DNA polymerases
⇒ Eukaryotic DNA polymerases
⇒ Replicons for DNA replication
⇒ DNA replication in prokaryotes 
⇒ Experimental approaches for the study of DNA replication
⇒ Initiation of DNA replication
⇒ Elongation of DNA chain
⇒ Replication fork movement
⇒ Termination of DNA replication
⇒ DNA replication in eukaryotes 
⇒ DNA replication and cell cycle
⇒ Replication origins and initiation of DNA replication (cis and trans-acting elements)
⇒ Comparison of initiation of DNA replication with transcription initiation
⇒ Different steps involved in eukaryotic DNA replication
⇒ Synthesis of telomeric DNA by telomerase
⇒ Models of DNA replication
⇒ Replication fork model
⇒ Rolling circle model of DNA replication
⇒ Mitochondrial DNA replication and D-loops
⇒ RNA directed DNA synthesis (reverse transcription)
⇒ DNA modification and DNA restriction
⇒ DNA repair
⇒ Excision repair systems in E. coli
⇒ An SOS repair system in E. coli
⇒ DNA repair and genetic diseases in humans


RNA primers in DNA replication
No DNA polymerase enzyme is known, which can initiate synthesis of DNA chain without the availability of a primer strand. Therefore, short segments of RNA are first synthesized before actual DNA replication starts. These RNA segments are later excised and filled with DNA with the help of enzyme DNA polymerase I. It was found that if RNA synthesis is shopped by a drug rifampicin, DNA replication stops thus proving the role of RNA primers in DNA replication (Fig. 26.12). There are several other evidences which established this point.
  
Discontinuous DNA replication using RNA primers ; at 5' ends of segments.
Fig. 26.12. Discontinuous DNA replication using RNA primers ; at 5' ends of segments.


 
     






     
     
 
 
     
 
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