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Section: Genetics » Chemistry of the Gene » Synthesis, Modification and Repair of DNA
 
 
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  Regulation of DNA replication by anti-sense RNA primer
 
     
 
Content
⇒ Chemistry of the Gene 2.  Synthesis, Modification and Repair of DNA
⇒ DNA replication: general features 
⇒ Semi-conservative DNA replication in E. coli
⇒ Semi-conservative replication of chromosomes in eukaryotes
⇒ Semi-discontinuous DNA replication
⇒ Unidirectional and bidirectional DNA replication
⇒ RNA primers in DNA replication
⇒ Regulation of DNA replication by anti-sense RNA primer
⇒ Prokaryotic DNA polymerases
⇒ Eukaryotic DNA polymerases
⇒ Replicons for DNA replication
⇒ DNA replication in prokaryotes 
⇒ Experimental approaches for the study of DNA replication
⇒ Initiation of DNA replication
⇒ Elongation of DNA chain
⇒ Replication fork movement
⇒ Termination of DNA replication
⇒ DNA replication in eukaryotes 
⇒ DNA replication and cell cycle
⇒ Replication origins and initiation of DNA replication (cis and trans-acting elements)
⇒ Comparison of initiation of DNA replication with transcription initiation
⇒ Different steps involved in eukaryotic DNA replication
⇒ Synthesis of telomeric DNA by telomerase
⇒ Models of DNA replication
⇒ Replication fork model
⇒ Rolling circle model of DNA replication
⇒ Mitochondrial DNA replication and D-loops
⇒ RNA directed DNA synthesis (reverse transcription)
⇒ DNA modification and DNA restriction
⇒ DNA repair
⇒ Excision repair systems in E. coli
⇒ An SOS repair system in E. coli
⇒ DNA repair and genetic diseases in humans
Regulation of DNA replication by anti-sense RNA primer
While studying DNA replication in an E. coli plasmid (ColEl), Jun-ichi Tomizawa in USA demonstrated that certain RNA molecules, synthesized on DNA strand complementary to the one that produces RNA primers, are actually synthesized. These were described as antisense inhibitor molecules and the rate of multiplication of the plasmid depended on the ratio of RNA primers to these antisense molecules. Obviously antisense RNA molecules hybridize with RNA primers and render them incapable of initiating DNA replication. Antisense RNA is also used for regulation of protein synthesis (gene expression) and has been shown to offer useful technology for therapeutic uses against certain diseases caused by viruses, etc. (for more details, consult Regulation of Gene Expression 1.  Operon Circuits in Bacteria and other Prokaryotes).

 
     






     
     
 
 
     
 
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