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Section: Genetics » Chemistry of the Gene » Synthesis, Modification and Repair of DNA
 
 
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  DNA replication in prokaryotes
 
     
 
Content
Chemistry of the Gene 2.  Synthesis, Modification and Repair of DNA
DNA replication: general features 
Semi-conservative DNA replication in E. coli
Semi-conservative replication of chromosomes in eukaryotes
Semi-discontinuous DNA replication
Unidirectional and bidirectional DNA replication
RNA primers in DNA replication
Regulation of DNA replication by anti-sense RNA primer
Prokaryotic DNA polymerases
Eukaryotic DNA polymerases
Replicons for DNA replication
DNA replication in prokaryotes 
Experimental approaches for the study of DNA replication
Initiation of DNA replication
Elongation of DNA chain
Replication fork movement
Termination of DNA replication
DNA replication in eukaryotes 
DNA replication and cell cycle
Replication origins and initiation of DNA replication (cis and trans-acting elements)
Comparison of initiation of DNA replication with transcription initiation
Different steps involved in eukaryotic DNA replication
Synthesis of telomeric DNA by telomerase
Models of DNA replication
Replication fork model
Rolling circle model of DNA replication
Mitochondrial DNA replication and D-loops
RNA directed DNA synthesis (reverse transcription)
DNA modification and DNA restriction
DNA repair
Excision repair systems in E. coli
An SOS repair system in E. coli
DNA repair and genetic diseases in humans
DNA Replication in Prokaryotes
Among prokaryotes, DNA replication in vitro has been studied in great detail in E. coli and its phages and plasmids. The techniques of genetics and biochemistry have been used to identify genes and their products (proteins) involved in DNA replication (Table 26.3). The most extensively studied examples include DNA replication from (i) E. coli chromosomal origin oriC; (ii) phage replication origin, oriλ; (iii) phage PI lysogenic origin oriP1; and (iv) ColE1 and pBR322 plasmid origin. Among these four systems, more variation is found in the initiation than in the elongation and termination of DNA replication.

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