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Section: Biotechnology Methods
 
 
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  Biotechnology Methods
 
     
 
Tools and Techniques in Biological Studies

 - Spectrophotometry

 - Electrophoresis

 - Column Chromatography

 - pH Meter

 - Centrifugation

 - Ultrasonic Cell Disruption

 - Conductivity Meter

 - Radioactive Tracers

 - Autoradiography

 - Photography

 - Statistics

 - Graphs

 - Computers



Biochemistry

 - Carbohydrates

 - Qualitative Tests

 - Qualitative Analysis

 - Estimate the Amount of Reducing Sugars

 - Estimation of Reducing Sugar by Somogyi’s Method

 - Estimation of Sugar by Folin-Wu Method

 - Estimation of Sugar by Hagedorn-Jenson Method

 - Estimation of Reducing Sugars by the Dinitro Salicylic Acid (DNS) Method

 - Determination of Blood Glucose by Hagedorn-Jenson Method

 - Determining Blood Sugar by Nelson and Somogyi’s Method

 - Determination of Blood Glucose by the O-Toluidine Method

 - Estimation of Protein by the Biuret Method

 - Estimation of Protein by the FC-Method

 - Protein Assay by Bradford Method

 - Estimation of Protein by the Lowry Protein Assay

 - Biuret Protein Assay

 - Estimation of DNA by the Diphenylamine Method

 - Estimation of RNA by the Orcinol Method



Enzymology

 - Enzymes

 - Demonstrating the Presence of Catalase in Pig’s Liver

 - Determining the Optimum pH for Trypsin

 - Extraction of Tyrosinase

 - Preparation of Standard Curve

 - Enzyme Concentration

 - Effects of pH

 - Effects of Temperature

 - Computer Simulation of Enzyme Activity

 - Kinetic Analysis

 - Determination of Km and Vmax

 - Addition of Enzyme Inhibitors

 - Protein Concentration/Enzyme Activity

 - Studying the Action and Activity of Amylase on Starch Digestion

 - Determination of the Effect of pH on the Activity of Human Salivary a-Amylase

 - Determining the Effect of Temperature on the Activity of Human Salivary a-Amylase

 - Construction of the Maltose Calibration Curve



Electrophoresis

 - Electrophoresis

 - Preparation of SDS-Polyacrylamide Gels

 - Separation of Protein Standards: SDS-PAGE

 - Coomassie Blue Staining of Protein Gels

 - Silver Staining of Gels

 - Documentation

 - Western Blots

 - Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE)

 - Preparation of Polyacrylamide Gels

 - Agarose Gel Electrophoresis
 
     





     
     
 
 
     
 
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