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Section: General Biotechnology / Genes & Genetic Engineering
 
 
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  Techniques of Genetic Engineering
 
     
 
Content

⇒ Gene cloning in prokaryotes

 

⇒ Isolation of DNA to be cloned 

 

⇒ Insertion of DNA fragment into vector 

   

⇒ Use of restriction Linkers

   

⇒ Use of homopolymer tails

 

⇒ Transfer of recombinant DNA into bacterial cells

 

⇒ Selection of clones

   

⇒ Colony hybridization techniques

   

⇒ In vitro translation technique

   

⇒ Immunological tests

   

⇒ Blotting Techniques

 

⇒ Recovery of cells

 

⇒ Expression of cloned DNA

   

⇒ Shine-Dalgano sequence

   

⇒ Expression vectors

⇒ Gene cloning in eukaryotes

 

⇒ Plant cells

   

⇒ Yeasts

   

⇒ Filamentous fungi

   

⇒ Agrobacterium plasmids

   

⇒ Plant cell transformation

   

⇒ Plant cell transformation by ultrasonication

   

⇒ Liposome mediated gene transfer

 

⇒ Animal cell  

   

⇒ Animal viruses

   

⇒ Electroporation

   

⇒ Particle bombardment
   

⇒ Microinjection

   

⇒ Direct transformation

⇒ Site directed mutagenesis

 

⇒ Methods of mutagenesis


 
     






     
     
 
 
     
 
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